AUTHOR=Iannelli Francesco , Santagati Maria , Santoro Francesco , Oggioni Marco R., Stefani Stefania , Pozzi Gianni 

TITLE=Nucleotide Sequence of Conjugative Prophage Φ1207.3 (formerly Tn1207.3) carrying the mef(A)/msr(D) genes for efflux resistance to macrolides in Streptococcus pyogenes

JOURNAL=Frontiers in Microbiology

VOLUME=5

YEAR=2014

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2014.00687

DOI=10.3389/fmicb.2014.00687

ISSN=1664-302X

ABSTRACT=<p>Genetic element Φ1207.3 (formerly Tn<italic>1207.3</italic>) is a prophage of <italic>Streptococcus pyogenes</italic> which carries the macrolide efflux resistance genes <italic>mef</italic>(A)/<italic>msr</italic>(D) and is capable of conjugal transfer among streptococci. Complete nucleotide sequence showed that Φ1207.3 is 52,491 bp in length and contained 58 open reading frames (ORFs). A manual homology-based annotation with functional prediction of the hypothetical gene product was possible only for 34 out of 58 ORFs. Φ1207.3 codes for two different C-methylation systems, several phage structural genes, a lysis cassette (composed by a holin and a peptidoglycan hydrolase), and three site-specific resolvases of the serine recombinase family.</p>