AUTHOR=Liu He , Yang Chun-Lan , Ge Meng-Yu , Ibrahim Muhammad , Li Bin , Zhao Wen-Jun , Chen Gong-You , Zhu Bo , Xie Guan-Lin 

TITLE=Regulatory role of tetR gene in a novel gene cluster of  Acidovorax avenae subsp. avenae RS-1 under oxidative stress

JOURNAL=Frontiers in Microbiology

VOLUME=5

YEAR=2014

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2014.00547

DOI=10.3389/fmicb.2014.00547

ISSN=1664-302X

ABSTRACT=<p><italic>Acidovorax avenae</italic> subsp. <italic>avenae</italic> is the causal agent of bacterial brown stripe disease in rice. In this study, we characterized a novel horizontal transfer of a gene cluster, including <italic>tetR</italic>, on the chromosome of <italic>A. avenae</italic> subsp. <italic>avenae</italic> RS-1 by genome-wide analysis. TetR acted as a repressor in this gene cluster and the oxidative stress resistance was enhanced in <italic>tetR</italic>-deletion mutant strain. Electrophoretic mobility shift assay demonstrated that TetR regulator bound directly to the promoter of this gene cluster. Consistently, the results of quantitative real-time PCR also showed alterations in expression of associated genes. Moreover, the proteins affected by TetR under oxidative stress were revealed by comparing proteomic profiles of wild-type and mutant strains via 1D SDS-PAGE and LC-MS/MS analyses. Taken together, our results demonstrated that <italic>tetR</italic> gene in this novel gene cluster contributed to cell survival under oxidative stress, and TetR protein played an important regulatory role in growth kinetics, biofilm-forming capability, superoxide dismutase and catalase activity, and oxide detoxicating ability.</p>