AUTHOR=Engelbrektson Anna , Hubbard Christopher G. , Tom Lauren M. , Boussina Aaron , Jin Yong T. , Wong Hayden , Piceno Yvette M. , Carlson Hans K. , Conrad Mark E. , Anderson Gary , Coates John D. TITLE=Inhibition of microbial sulfate reduction in a flow-through column system by (per)chlorate treatment JOURNAL=Frontiers in Microbiology VOLUME=5 YEAR=2014 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2014.00315 DOI=10.3389/fmicb.2014.00315 ISSN=1664-302X ABSTRACT=
Microbial sulfate reduction is a primary cause of oil reservoir souring. Here we show that amendment with chlorate or perchlorate [collectively (per)chlorate] potentially resolves this issue. Triplicate packed columns inoculated with marine sediment were flushed with coastal water amended with yeast extract and one of nitrate, chlorate, or perchlorate. Results showed that although sulfide production was dramatically reduced by all treatments, effluent sulfide was observed in the nitrate (10 mM) treatment after an initial inhibition period. In contrast, no effluent sulfide was observed with (per)chlorate (10 mM). Microbial community analyses indicated temporal community shifts and phylogenetic clustering by treatment. Nitrate addition stimulated