AUTHOR=Rosano Germán L. , Ceccarelli Eduardo A. 

TITLE=Recombinant protein expression in Escherichia coli: advances and challenges

JOURNAL=Frontiers in Microbiology

VOLUME=5

YEAR=2014

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2014.00172

DOI=10.3389/fmicb.2014.00172

ISSN=1664-302X

ABSTRACT=<p><italic>Escherichia coli</italic> is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies. We review the different approaches for the synthesis of recombinant proteins in <italic>E. coli</italic> and discuss recent progress in this ever-growing field.</p>