AUTHOR=Sawabe Tomoo , Ogura Yoshitoshi , Matsumura Yuta , Gao Feng , Amin AKM R., Mino Sayaka , Nakagawa Satoshi , Sawabe Toko , Kumar Ramesh , Fukui Yohei , Satomi Masataka , Matsushima Ryoji , Thompson Fabiano L., Gomez-Gil Bruno , Christen Richard , Maruyama Fumito , Kurokawa Ken , Hayashi Testuya 

TITLE=Updating the Vibrio clades defined by multilocus sequence phylogeny: proposal of eight new clades, and the description of Vibrio tritonius sp. nov.

JOURNAL=Frontiers in Microbiology

VOLUME=4

YEAR=2013

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2013.00414

DOI=10.3389/fmicb.2013.00414

ISSN=1664-302X

ABSTRACT=<p>To date 142 species have been described in the <italic>Vibrionaceae</italic> family of bacteria, classified into seven genera; <italic>Aliivibrio</italic>, <italic>Echinimonas</italic>, <italic>Enterovibrio</italic>, <italic>Grimontia</italic>, <italic>Photobacterium</italic>, <italic>Salinivibrio</italic> and <italic>Vibrio</italic>. As vibrios are widespread in marine environments and show versatile metabolisms and ecologies, these bacteria are recognized as one of the most diverse and important marine heterotrophic bacterial groups for elucidating the correlation between genome evolution and ecological adaptation. However, on the basis of 16S rRNA gene phylogeny, we could not find any robust monophyletic lineages in any of the known genera. We needed further attempts to reconstruct their evolutionary history based on multilocus sequence analysis (MLSA) and/or genome wide taxonomy of all the recognized species groups. In our previous report in 2007, we conducted the first broad multilocus sequence analysis (MLSA) to infer the evolutionary history of vibrios using nine housekeeping genes (the 16S rRNA gene, <italic>gapA</italic>, <italic>gyrB</italic>, <italic>ftsZ</italic>, <italic>mreB, pyrH</italic>, <italic>recA</italic>, <italic>rpoA</italic>, and <italic>topA</italic>), and we proposed 14 distinct clades in 58 species of <italic>Vibrionaceae</italic>. Due to the difficulty of designing universal primers that can amplify the genes for MLSA in every <italic>Vibrionaceae</italic> species, some clades had yet to be defined. In this study, we present a better picture of an updated molecular phylogeny for 86 described vibrio species and 10 genome sequenced <italic>Vibrionaceae</italic> strains, using 8 housekeeping gene sequences. This new study places special emphasis on (1) eight newly identified clades (<italic>Damselae</italic>, <italic>Mediterranei</italic>, <italic>Pectenicida</italic>, <italic>Phosphoreum</italic>, <italic>Profundum</italic>, <italic>Porteresiae</italic>, <italic>Rosenbergii</italic>, and <italic>Rumoiensis</italic>); (2) clades amended since the 2007 proposal with recently described new species; (3) orphan clades of genomospecies F6 and F10; (4) phylogenetic positions defined in 3 genome-sequenced strains (N418, EX25, and EJY3); and (5) description of <italic>V. tritonius</italic> sp. nov., which is a member of the “<italic>Porteresiae</italic>” clade.</p>