AUTHOR=Reyes Luis H., Winkler James , Kao Katy C.

TITLE=Visualizing evolution in real-time method for strain engineering

JOURNAL=Frontiers in Microbiology

VOLUME=3

YEAR=2012

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2012.00198

DOI=10.3389/fmicb.2012.00198

ISSN=1664-302X

ABSTRACT=<p>The adaptive landscape for an industrially relevant phenotype is determined by the effects of the genetic determinants on the fitness of the microbial system. Identifying the underlying adaptive landscape for a particular phenotype of interest will greatly enhance our abilities to engineer more robust microbial strains. Visualizing evolution in real-time (VERT) is a recently developed method based on <italic>in vitro</italic> adaptive evolution that facilitates the identification of fitter mutants throughout the course of evolution. Combined with high-throughput genomic tools, VERT can greatly enhance the mapping of adaptive landscapes of industrially relevant phenotypes in microbial systems, thereby expanding our knowledge on the parameters that can be used for strain engineering.</p>