AUTHOR=Dandekar Thomas , Fieselmann Astrid , Popp Jasmin , Hensel Michael 

TITLE=Salmonella enterica: a surprisingly well-adapted intracellular lifestyle

JOURNAL=Frontiers in Microbiology

VOLUME=3

YEAR=2012

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2012.00164

DOI=10.3389/fmicb.2012.00164

ISSN=1664-302X

ABSTRACT=<p>The infectious intracellular lifestyle of <italic>Salmonella enterica</italic> relies on the adaptation to nutritional conditions within the <italic>Salmonella</italic>-containing vacuole (SCV) in host cells. We summarize latest results on metabolic requirements for <italic>Salmonella</italic> during infection. This includes intracellular phenotypes of mutant strains based on metabolic modeling and experimental tests, isotopolog profiling using <sup>13</sup>C-compounds in intracellular <italic>Salmonella</italic>, and complementation of metabolic defects for attenuated mutant strains towards a comprehensive understanding of the metabolic requirements of the intracellular lifestyle of <italic>Salmonella</italic>. Helpful for this are also genomic comparisons. We outline further recent studies and which analyses of intracellular phenotypes and improved metabolic simulations were done and comment on technical required steps as well as progress involved in the iterative refinement of metabolic flux models, analyses of mutant phenotypes, and isotopolog analyses. <italic>Salmonella</italic> lifestyle is well-adapted to the SCV and its specific metabolic requirements. <italic>Salmonella</italic> metabolism adapts rapidly to SCV conditions, the metabolic generalist <italic>Salmonella</italic> is quite successful in host infection.</p>