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ORIGINAL RESEARCH article
Front. Med.
Sec. Pulmonary Medicine
Volume 12 - 2025 | doi: 10.3389/fmed.2025.1545095
Identification and Validation of Differentially Expressed Genes in Allergic Asthma Pathogenesis Using Whole-Transcriptome Sequencing
Provisionally accepted- Yanan University Affiliated Hospital, Yanan, China
Objective: This study aims to systematically identify differential gene expression profiles in patients with allergic asthma through whole-transcriptome sequencing and validate the role of these genes in asthma pathogenesis, thereby uncovering potential molecular mechanisms.Methods: This study recruited a cohort of eighty individuals diagnosed with allergic asthma and forty healthy controls. RNA was extracted from both peripheral blood and airway samples, and sequencing was performed using the Illumina NovaSeq 6000 platform. Potential differential genes were confirmed through three independent techniques to validate the findings: quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry, and Western blot analysis. Differential gene expression was analyzed using DESeq2 software, providing a rigorous statistical framework for RNA-Seq data interpretation. Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were employed to Conclusion: Differential gene expression profiles play a crucial role in the pathogenesis of asthma.This study provides important evidence for understanding the molecular mechanisms of asthma and developing novel targeted therapeutic strategies.
Keywords: Allergic Asthma, whole-transcriptome sequencing, Differentially expressed genes, Functional enrichment analysis, Asthma pathogenesis
Received: 14 Dec 2024; Accepted: 21 Mar 2025.
Copyright: © 2025 Cui, Liu, Wang, He and Xu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Yanxia Xu, Yanan University Affiliated Hospital, Yanan, China
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