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SYSTEMATIC REVIEW article
Front. Med.
Sec. Infectious Diseases: Pathogenesis and Therapy
Volume 12 - 2025 | doi: 10.3389/fmed.2025.1512345
Evaluation of the NG-Test Carba 5 for the clinical detection of carbapenemaseproducing gram-negative bacteria
Provisionally accepted
Hai-Feng Qin * Jin-Ke He * Xin Chen * Ke Jiang * Xiao- Yan Cai * Xiao-Ni Wu * Lei Ye * Hao-Kai Chen * Yong Xia *
Xu-Guang Guo *- Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Background: Currently, the spread and prevalence of carbapenem-resistant gramnegative bacteria cause a worldwide health problem, significantly affecting patients' prognosis. Therefore, reliable detection of carbapenemases is crucial for managing and controlling infections. Numerous investigations have shown that the innovative immunochromatographic assay NG-Test Carba 5 has great sensitivity and specificity for carbapenemase typing. This meta-analysis aimed to comprehensively assess the efficacy of the NG-Test Carba 5 in the clinical detection of carbapenemase-producing gram-negative bacteria. Methods: Previously published articles were systematically reviewed, relevant data were extracted, and the results were pooled and analyzed using Meta-DiSc 1.4 and Stata 12.0 software. Results: The sensitivity, specificity, positive LR value, and negative LR value for the identification of carbapenemase-type KPC, NDM, VIM, IMP, and OXA-48-like by immunochromatographic NG-Test Carba 5 using PCR as gold standard were 0.97 (95% CI (0.97, 0.98)), 0.99 (95% CI (0.99, 1.00)), 65.38 (95% CI (36.73, 116.39)), and 0.03 (95% CI (0.02, 0.05)), respectively, and the combined diagnostic odds ratio was 2,734.42 (95% CI (1,464.05, 5,107.12)). The AUC of the SROC curve was 0.9976. Conclusions: In summary, the NG-Test Carba 5 is a clinical test that can identify and quickly detect five major carbapenemases, thus offering valuable insights for clinical decision-making and infection control.
Keywords: NG-Test Carba 5, Immunochromatographic assay, Carbapenemase-producing, carbapenem-resistant Gram negative bacteria, Clinical detection
Received: 21 Oct 2024; Accepted: 14 Feb 2025.
Copyright: © 2025 Qin, He, Chen, Jiang, Cai, Wu, Ye, Chen, Xia and Guo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hai-Feng Qin, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Jin-Ke He, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Xin Chen, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Ke Jiang, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Xiao- Yan Cai, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Xiao-Ni Wu, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Lei Ye, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Hao-Kai Chen, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Yong Xia, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Xu-Guang Guo, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.