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ORIGINAL RESEARCH article
Front. Med.
Sec. Dermatology
Volume 11 - 2024 |
doi: 10.3389/fmed.2024.1453940
This article is part of the Research Topic Advances and Novel Intervention in Photodermatology View all articles
Cell subset difference of human sun-exposed and un-exposed skin： preliminary single cell sequencing and biological analysis from a single case
Provisionally accepted
Zhou Feng
1
Sun Yu
1*
Chen Xinling
1
Hou Wenyi
1*
Chen Haiyan
1*
Lai Wei
1*
Xu Qingfang
1*
Shen Jing
2*
Han Kai
2*
Yue Zheng
1,2*- 1 Department of Dermato-venereology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
- 2 Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, China
The composition and subsets of skin cells continuously change in a dynamic manner. However, the specific microcosmic alterations of human photoaged skin, independent of chronologic aging, remain unclear and have been infrequently analyzed. This study aimed to evaluate biological processes and mechanisms underlying cell-subgroup alterations in skin photoaging by utilizing single cell sequencing and biological analysis from a single case. The investigation compared sun-exposed forearm skin and unexposed buttock skin from the same individual, obtained through skin punch biopsy. Our analysis identified 25 cell clusters and 12 skin cell types , revealing significant changes in unique gene expressions. A comparison of cell numbers within each cluster revealed 9 dominant cell clusters in sun-exposed skin and 16 in unexposed skin. Enrichment analysis indicated that PD-L1 expression and the PD-1 checkpoint pathway were more prominent in sun-exposed skin, while MAPK, TNF-alpha , TGF−beta and apoptosis pathways were more enriched in hair follicle cells of sun-exposed skin. This study uncovers changes in cell components in photoaged skin from a single case and provides novel insights into cellular subpopulations and pathology during the repeated UVA-induced skin damage. These findings enhance our understanding of the complex interplay between different cells in photoaged skin and offer potential targets for preventing human skin photoaging and UV-induced skin cancers.
Keywords: UV, Skin, Aging, Photoaging, Single cell sequencing
Received: 24 Jun 2024; Accepted: 23 Sep 2024.
Copyright: © 2024 Feng, Yu, Xinling, Wenyi, Haiyan, Wei, Qingfang, Jing, Kai and Zheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Sun Yu, Department of Dermato-venereology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
Hou Wenyi, Department of Dermato-venereology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
Chen Haiyan, Department of Dermato-venereology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
Lai Wei, Department of Dermato-venereology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
Xu Qingfang, Department of Dermato-venereology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
Shen Jing, Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, China
Han Kai, Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, China
Yue Zheng, Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, China
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