Skip to main content

ORIGINAL RESEARCH article

Front. Med.
Sec. Hepatobiliary Diseases
Volume 11 - 2024 | doi: 10.3389/fmed.2024.1434156

Bioinformatic Analysis of Long Non-Coding RNA AC004816.1 and Its Independent Association with Poor Prognosis and Interactions with Danzhi Xiaoyaosan

Provisionally accepted
Shengkai Wang Shengkai Wang 1Hangfei Wu Hangfei Wu 2Suqin Hu Suqin Hu 3Ruifan Ye Ruifan Ye 4*
  • 1 Zhejiang Cancer Hospital, Hangzhou, Jiangsu Province, China
  • 2 Zhejiang Zhuji People's Hospital, Zhuji, China
  • 3 Ningbo Diagnostic Pathology Center, Ningbo, Zhejiang Province, China
  • 4 First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China

The final, formatted version of the article will be published soon.

    Objective: To investigate the relationship between AC004816.1 expression and clinical characteristics in hepatocellular carcinoma (HCC) patients. Methods: We analysed long non-coding RNA (lncRNA) AC004816.1 expression using data from The Cancer Genome Atlas (TCGA). A chi-square (χ2) test assessed its relationship with clinicopathological features. We performed receiver operating characteristic (ROC), Cox regression, and Kaplan–Meier analyses for diagnostic and prognostic significance. Gene set enrichment analysis (GSEA) was conducted, and interactions with infiltrating immune cells were evaluated using the ESTIMATE method. We also constructed a compound-target-lncRNA network based on the Traditional Chinese Medicine Systems Pharmacology database, exploring how Danzhi Xiaoyaosan might influence AC004816.1 expression. Results: The expression of AC004816.1 mRNA in HCC tissues was elevated and was associated with poor overall survival (OS), shorter progress-free intervals and poor disease-specific survival (all P < 0.05). The area under the curve (AUC) value for AC004816.1 mRNA expression levels was 0.958. With regards to diagnostic value at varying stages, the findings revealed a comparable diagnostic significance, with AUC values of 0.968, 0.906, 0.926 and 0.828 for stages I, II, III and IV, respectively. Furthermore, univariate analysis showed that elevated AC004816.1 mRNA levels, T classification and advanced stage correlated with OS outcomes. Regression analysis showed that AC004816.1 mRNA levels are important risk factors for OS outcomes in HCC. Furthermore, a visualisation network was constructed, which indicated that AC004816.1 has three overlapping target genes with Danzhi Xiaoyaosan. Conclusion: Elevated AC004816.1 mRNA in HCC is associated with poor OS outcomes, suggesting its potential as an early diagnostic and prognostic marker for HCC.

    Keywords: AC004816.1, Hepatocellular Carcinoma, Traditional Chinese Medicine, bioinformatics, Danzhi Xiaoyaosan

    Received: 17 May 2024; Accepted: 07 Oct 2024.

    Copyright: © 2024 Wang, Wu, Hu and Ye. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Ruifan Ye, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.