AUTHOR=Mao Qiang , Shao Weijie , Lv Shuaijie , Tong Peijian , He Bangjian 

TITLE=Case report: Directional infusion of peripheral blood stem cells into the necrotic zone in femoral heads through the medial circumflex femoral artery: A tracing study

JOURNAL=Frontiers in Medicine

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2022.945268

DOI=10.3389/fmed.2022.945268

ISSN=2296-858X

ABSTRACT=<sec><title>Objective</title><p>This study aimed to explore whether peripheral blood stem cells (PBSCs) infused through the medial circumflex femoral artery to treat osteonecrosis of the femoral head (ONFH) could migrate into the necrotic area of femoral head.</p></sec><sec><title>Methods</title><p>We collected PBSCs from a patient who had bilateral ONFH by apheresis technique using COBE spectra apheresis system (COBE BCT Inc, Lakewood, CO, USA) after subcutaneous injections of granulocyte-colony stimulating factor (G-CSF) at a dosage of 10 μg/kg for 4 days to mobilize PBSCs. After that, 100 MBq 2-[<sup>18</sup>F]-fluoro-2-deoxy-D-glucose (<sup>18</sup>F-FDG) was used to label PBSCs. <sup>18</sup>F-FDG labeled PBSCs were infused into the left femoral head <italic>via</italic> the medial circumflex femoral artery to treat ONFH. Then the patient was underwent three-dimensional positron emission tomography (3D-PET) examination 60 min after cell infusion to monitor the biological distribution of <sup>18</sup>F-FDG-labeled PBSCs, and to observe whether the transplanted PBSCs could migrate into the necrotic area of femoral head.</p></sec><sec><title>Results</title><p>The total number of monouclear cells in the peripheral blood stem cell suspension was 1.95 × 10<sup>8</sup> which contained 2.20 × 10<sup>6</sup> CD34<sup>+</sup> cells. The activity of <sup>18</sup>F-FDG in the labeled cells was 1.8Bq/10<sup>3</sup> monouclear cells. 3D-PET imaging showed that <sup>18</sup>F-FDG radioactivity was detected in the necrotic area of femoral head, acetabulum and femoral bone marrow cavity after transplantation of <sup>18</sup>F-FDG-labeled PBSCs via the medial circumflex femoral artery. It is worth noting that although PBSCs labeled with <sup>18</sup>F-FDG were widely distributed around the hip, such as femoral bone marrow cavity, femoral head and acetabulum, PBSCs were generally located in the necrotic area of femoral head.</p></sec><sec><title>Conclusions</title><p>PBSCs could enter into the femoral head and migrate into the necrotic field of femoral head participating in the repair of osteonecrosis after infusion through the medial circumflex femoral artery.</p></sec>