AUTHOR=Xing Fanfan , Lo Simon K. F. , Ma Yuanchao , Ip Jonathan Daniel , Chan Wan-Mui , Zhou Meixun , Gong Miaozi , Lau Susanna K. P. , Woo Patrick C. Y. 

TITLE=Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report

JOURNAL=Frontiers in Medicine

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2022.824122

DOI=10.3389/fmed.2022.824122

ISSN=2296-858X

ABSTRACT=<p>We present the first report of histology- and culture-proven <italic>Mycobacterium marinum</italic> infection diagnosed by next-generation sequencing (NGS). It took &lt;2 days to make a microbiological diagnosis using the Oxford Nanopore Technologies' MinION device, compared to 20 days for the mycobacterium to be isolated from the tissue biopsy. NGS is particularly useful for culture-negative and slow-growing microorganism infections, such as mycobacterial, fungal and partially treated pyogenic bacterial infections. Due to its low equipment cost, short turn-around-time and portable size, the Oxford Nanopore Technologies' MinION device is a useful platform for NGS in routine clinical microbiology laboratories.</p>