AUTHOR=Zhang Xinyuan , Qiu Bingjie , Wang Qiyun , Sivaprasad Sobha , Wang Yanhong , Zhao Lin , Xie Rui , Li Lei , Kang Wenting 

TITLE=Dysregulated Serum Lipid Metabolism Promotes the Occurrence and Development of Diabetic Retinopathy Associated With Upregulated Circulating Levels of VEGF-A, VEGF-D, and PlGF

JOURNAL=Frontiers in Medicine

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2021.779413

DOI=10.3389/fmed.2021.779413

ISSN=2296-858X

ABSTRACT=<p><bold>Purpose:</bold> This study aims to explore the correlations of arteriosclerosis-associated plasma indices with various severity levels of diabetic retinopathy (DR) and to test the hypothesis that elevated circulating level of known angiogenic cytokines induced by hyperglycemia is associated with dyslipidemia on DR.</p><p><bold>Methods:</bold> This cross-sectional study consists of 131 patients with type 2 diabetes. The patients were categorized based on their DR status into those with no DR (diabetes mellitus, DM), non-proliferative diabetic retinopathy (NPDR), and proliferative diabetic retinopathy (PDR) groups. The biochemical profile including fasting glucose, glycated hemoglobin (HbA1c), lipid profile were estimated, plasma angiogenic cytokines (vascular endothelial growth factor, VEGF-A, -C, -D) and placental growth factor (PlGF) were analyzed by protein microarrays. The atherogenic plasma index (API) was defined as low-density lipoprotein cholesterol/high-density lipoprotein cholesterol (LDL-C/HDL-C); atherogenic index (AI) was calculated as (TC-(HDL-C))/HDL-C and atherogenic index of plasma (AIP) was defined as log (TG/HDL-C).</p><p><bold>Results:</bold> No significant differences were detected in the duration of hypertension, age, and gender between the three groups. Serum TC and LDL-C, AI, and API in the NPDR group and PDR group were significantly higher than those in the DM group. The circulating level of PlGF, VEGF-A, and VEGF-C were significantly correlated with the severity of DR. VEGF-D is a risk factor independent of API (<italic>Z</italic> = −2.61, <italic>P</italic> = 0.009) and AI (<italic>Z</italic> = −2.40, <italic>P</italic> = 0.016). Multivariate logistic regression showed that AI and API are strong risk factors for the occurrence and severity of DR. Associated with AI and API, VEGF-D and PlGF contribute to DR: VEGF-D [AI: <italic>P</italic> = 0.038, odd ratio (OR) = 1.38; VEGF-D: <italic>P</italic> = 0.002, OR = 1.00. API: <italic>P</italic> = 0.027, OR = 1.56, VEGF-D:<italic>P</italic> = 0.002, OR = 1.00] and PlGF [AI: <italic>P</italic> = 0.021, OR = 1.43; VEGF-D: <italic>P</italic> = 0.004, OR = 1.50. API: <italic>P</italic> = 0.011, OR = 1.66; VEGF-D: <italic>P</italic> = 0.005, OR = 1.49].</p><p><bold>Conclusions:</bold> Total cholesterol (TC) and LDL-C are risk factors for presence of any DR. Atherogenic index and API are novel and better predictive indicators for the occurrence and severity of DR in comparion with the traditional lipid profiles. Abnormal lipid metabolism are associated with the upregulation of circulating cytokines that are linked to the severity of DR.</p>