AUTHOR=Dong Li , Li Yi Fan , Jiang Xue , Lan Yin Jun , Shao Lei , Jonas Jost B. , Wei Wen Bin 

TITLE=In vivo Imaging of Retina and Choroid in Guinea Pigs

JOURNAL=Frontiers in Medicine

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2021.730494

DOI=10.3389/fmed.2021.730494

ISSN=2296-858X

ABSTRACT=<p><bold>Purpose:</bold> To evaluate the feasibility of <italic>in-vivo</italic> imaging of the retina and choroid using spectral domain optical coherence tomography (OCT) in guinea pigs.</p><p><bold>Methods:</bold> The study included 19 pigmented guinea pigs (age: 3–4 weeks) which underwent sonographic axial length measurements and OCT imaging. At study end, the animals were sacrificed and histomorphometric examinations of the retina and choroid were performed. We assessed the reproducibility of the OCT measurements and compared <italic>in-vivo</italic> measurements to histomorphometric data.</p><p><bold>Results:</bold> The mean thickness of the retina and choroid near the optic nerve head was 175.6 ± 25.8 and 63.4 ± 16.5 μm, respectively, and mean Bruch's membrane opening (BMO) diameter was 831 ± 121 μm. The intra-observer comparison of measurements of retinal thickness (intraclass correlation coefficient (ICC) = 0.92, 95% CI: 0.86–0.96; <italic>P</italic> &lt; 0.001), choroidal thickness (ICC = 0.92, 95% CI: 0.86–0.96; <italic>P</italic> &lt; 0.001), and BMO diameter (ICC = 0.92, 95% CI: 0.86–0.96; <italic>P</italic> &lt; 0.001) showed a high correlation. A high agreement was present also for the inter-observer reproducibility of the measurements of retinal thickness (Pearson correlation coefficient (<italic>R</italic>) = 0.98; <italic>P</italic> &lt; 0.001), choroidal thickness (<italic>R</italic> = 0.96; <italic>P</italic> &lt; 0.001), and BMO diameter (<italic>R</italic> = 0.98; <italic>P</italic> &lt; 0.001). The Bland-Altman plots showed that 2.6% (1/38), 5.3% (2/38), and 7.9% (3/38) of the measurement points of retinal thickness, choroidal thickness and BMO diameter, respectively, were located outside of the 95% limits of agreement. The OCT-based thickness measurements of retina and choroid were significantly higher than those measured by histomorphometry (both <italic>P</italic>-values &lt;0.01).</p><p><bold>Conclusion:</bold> OCT-based <italic>in-vivo</italic> morphometric imaging of the retina and choroid in guinea pigs is feasible with an acceptable intra-observer repeatability and inter-observer reproducibility.</p>