AUTHOR=Jiao Mengfan , Deng Xiang , Yang Hongfu , Dong Junqiang , Lv Jun , Li Fang 

TITLE=Case Report: A Severe and Multi-Site Nocardia farcinica Infection Rapidly and Precisely Identified by Metagenomic Next-Generation Sequencing

JOURNAL=Frontiers in Medicine

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2021.669552

DOI=10.3389/fmed.2021.669552

ISSN=2296-858X

ABSTRACT=<p><italic>Nocardia</italic> genus is an aerobic, gram-positive, and opportunistic pathogen, which mainly affects cell-mediated immunosuppressed patients. Early diagnosis and treatment greatly improve prognosis. However, the limitation of golden standard-bacterial culture exists. Here, we report a 61-year-old male with pneumonia, sepsis and intermuscular abscesses induced by <italic>Nocardia farcinica</italic>. Venous blood culture reported negative results. Former improper diagnosis and treatment did not improve his condition. With the assistant of metagenomic next-generation sequencing, the pathogen was identified as <italic>Nocardia farcinica</italic>. He was then applied with accurate treatment and had a remarkable clinical and radiological improvement.</p>