AUTHOR=Gu Ji-Na , Chen Lin , Weng Xing-Bei , Yang Xiao-Yan , Pan Dan-Mei 

TITLE=Clinical and Microbiological Characteristics of a Community-Acquired Carbapenem-Resistant Escherichia coli ST410 Isolate Harbouring blaNDM-5-Encoding IncX3-Type Plasmid From Blood

JOURNAL=Frontiers in Medicine

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2021.658058

DOI=10.3389/fmed.2021.658058

ISSN=2296-858X

ABSTRACT=<p><bold>Objectives:</bold> The aim of this research was to investigate the clinical and microbiological characteristics of a case of community-acquired carbapenem-resistant <italic>Escherichia coli</italic> isolated from a patient with a bloodstream infection in China.</p><p><bold>Methods:</bold><italic>Escherichia coli</italic> Huamei202001 was recovered from the first blood culture from a patient hospitalised in China. An antimicrobial susceptibility test was performed, and the genome was sequenced on an Illumina HiSeq X 10 platform with a 150-bp paired-end approach. The generated sequence reads were assembled using Unicycler, and the whole genome sequence data were analysed using bioinformatics tools. Moreover, the patient and her main family members obtained a faecal sample screening test for CRE, the positive strain was further isolated and the identification and antimicrobial susceptibility testing was performed.</p><p><bold>Results:</bold><italic>Escherichia coli</italic> Huamei202001 belonged to sequence type 410. In addition, a <italic>bla</italic>NDM-5-encoding IncX3-type plasmid was responsible for the spreading of carbapenem resistance. Only the patient was detected as having a positive faecal sample screening test for CRE. Strain Fec01 was identified as <italic>E. coli</italic>, and the antibiotic susceptibility profile was the same as that of <italic>E. coli</italic> Huamei202001.</p><p><bold>Conclusions:</bold><italic>Escherichia coli</italic> Huamei202001 is defined as community-acquired carbapenem-resistant Enterobacteriaceae. The clone ST410 that harbours the <italic>bla</italic>NDM-5-encoding IncX3-type plasmid is causing new high-risk clones globally. Thus, infection control measures should be strengthened to curb the dissemination of IncX3.</p>