AUTHOR=Jin Yanqi , Wang Zhouhan , Zhu Chunxia , Yang Qing , Lu Yingfeng , Yu Xiaopeng , Hong Bao , Wang Xiaojing , Zhang Yimin 

TITLE=Case Report: Proven Diagnosis of Culture-Negative Chronic Disseminated Candidiasis in a Patient Suffering From Hematological Malignancy: Combined Application of mNGS and CFW Staining

JOURNAL=Frontiers in Medicine

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2021.627166

DOI=10.3389/fmed.2021.627166

ISSN=2296-858X

ABSTRACT=<p>Chronic disseminated candidiasis (CDC) is a severe complication with high morbidity and mortality in patients with hematological malignancies who have undergone chemotherapy. Blood or sterile liver biopsy cultures are negative due to recurrent empirical antifungal therapy. With the escalating resistance to azole-based antifungal drugs in infection by <italic>Candida</italic> species, pathogen identification is becoming increasingly important for determining definitive diagnosis and treatment strategy. In this case report, we present, for the first time, diagnostic confirmation of a culture-negative CDC case with <italic>Candida tropicalis</italic> infection using a combination of metagenomics next-generation sequencing and calcofluor white staining.</p>