AUTHOR=Zhang Jie , Liu Zheng , Chang Cunyi , Hu Ming , Teng Yang , Li Jinjing , Zhang Xiangyu , Chi Yanxia 

TITLE=Ultrasound Imaging and Antithrombotic Effects of PLA-Combined Fe3O4-GO-ASA Multifunctional Nanobubbles

JOURNAL=Frontiers in Medicine

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2021.576422

DOI=10.3389/fmed.2021.576422

ISSN=2296-858X

ABSTRACT=<p>PLA-combined ferroferric oxide–graphene oxide–aspirin (Fe<sub>3</sub>O<sub>4</sub>-GO-ASA) multifunctional nanobubbles were prepared using the double emulsion-solvent evaporation method. The obtained composite nanobubbles had a regular spherical shape, Zeta potential of (−36.5 ± 10.0) mV, and particle size distribution range of 200–700 nm. The experimental results showed that PLA-combined Fe<sub>3</sub>O<sub>4</sub>-GO-ASA nanobubbles could effectively improve the antithrombin parameters of PT, TT, APTT, and INR, and significantly inhibit thrombosis when the composite nanobubbles with a concentration of 80 mg·mL<sup>−1</sup> interacted with the rabbit blood. The prepared composite nanobubbles could reach a significant ultrasonic imaging effect and good magnetic targeting under the magnetic field when the nanobubbles' concentration was only 60 mg·mL<sup>−1</sup>.</p>