AUTHOR=López Patricia , Rodríguez-Carrio Javier , Caminal-Montero Luis , Suárez Ana TITLE=Relationship Between T-Cell Exosomes and Cellular Subsets in SLE According to Type I IFN-Signaling JOURNAL=Frontiers in Medicine VOLUME=7 YEAR=2020 URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2020.604098 DOI=10.3389/fmed.2020.604098 ISSN=2296-858X ABSTRACT=

Objective: To quantify the levels of circulating exosomes derived from T-cells and monocytes and their possible associations with leukocyte subpopulations and cytokine milieu in Systemic Lupus Erythematosus (SLE).

Methods: Total circulating exosomes (CD9⁺-Ex) and those derived from T-cells (CD3⁺-Ex) and monocytes (CD14⁺-Ex) were quantified by flow cytometry in 82 SLE patients and 32 controls. Leukocyte subsets and serum cytokines were analyzed by flow cytometry or by immunoassays. IFN-score was evaluated by real time RT-PCR in whole blood samples from a subgroup of 73 patients and 24 controls.

Results: Activation markers (IFNR1 and BLyS) on monocytes, neutrophils and B-cells correlated inversely with circulating exosomes (CD9⁺-Ex, CD3⁺-Ex, and CD14⁺-Ex) in controls but directly with CD3⁺-Ex in patients (all p < 0.05). Although CD9⁺-Ex were increased in SLE, no differences were found in CD3⁺-Ex, supporting that exosome content accounts for this opposite role. Interestingly, CD4⁺CD28^null cells correlated with CD3⁺-Ex in patients and controls, and displayed similar associations with leukocyte subsets in both groups. Additionally, CD3⁺-Ex correlated in patients with the expression of CD25 in CD4⁺CD28^null cells. Furthermore, the activated status of this senescent subset was related to IFNα serum levels in controls and to IFN-score in SLE patients. Finally, patients presenting high IFN-score, in addition to elevated CD25⁺CD28^null cells associated with the activation of myeloid cells, displayed higher levels of inflammatory cytokines and chemokines.

Conclusion: Our results support a relationship between T-cell exosomes and cellular subsets in SLE according to type I IFN-signaling, which could amplify chronic immune activation and excessive cytokine/chemokine response.