AUTHOR=Resende Lucilene Aparecida , Aguiar-Soares Rodrigo Dian de Oliveira , Moreira Nádia das Dores , Ferreira Sidney de Almeida , Lanna Mariana Ferreira , Cardoso Jamille Mirelle de Oliveira , Mathias Fernando Augusto Siqueira , Coura-Vital Wendel , Mariano Reysla Maria da Silveira , Leite Jaqueline Costa , Silveira Patricia , de Carvalho Tatiane Furtado , Santos Renato Lima , Silveira-Lemos Denise da , Martins-Filho Olindo Assis , Dutra Walderez Ornelas , Reis Alexandre Barbosa , Giunchetti Rodolfo Cordeiro TITLE=In vitro Infectivity of Strains Isolated From Dogs Naturally Infected With Leishmania infantum Present a Distinct Pathogenic Profile in Hamsters JOURNAL=Frontiers in Medicine VOLUME=7 YEAR=2020 URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2020.00496 DOI=10.3389/fmed.2020.00496 ISSN=2296-858X ABSTRACT=

Visceral leishmaniasis (VL) is a severe disease caused by Leishmania infantum. Dogs are the parasite's main reservoir, favoring its transmission in the urban environment. The analysis of L. infantum from infected dogs contributes to the identification of more virulent parasites, thereby supporting basic and applied studies such as vaccinal and therapeutic strategies. We proposed the in vitro and in vivo characterization of L. infantum strains from naturally infected dogs from a VL endemic area based on an infectivity and pathogenicity analysis. DH82 canine macrophages were infected in vitro with different strains for infectivity analysis, showing distinct infectivity profiles. The strains that showed greater and lesser infectivity using in vitro analyses (616 and 614, respectively) were used to infect hamsters for pathogenicity analysis. The group infected with strain 616 showed 100% survival while the group infected with strain 614 showed 50% after seven months of follow up. Furthermore, the 614 strain induced more noticeable clinicopathological changes and biochemical abnormalities in liver function, along with high inflammation and parasite load in the liver and spleen. We confirmed high variability of infectivity and pathogenicity in L. infantum strains from infected dogs. The results support the belief that screening for L. infantum infectivity using in vitro experiments is inadequate when it comes to selecting the most pathogenic strain.