AUTHOR=Cui Ze-Hua , Jia Ling , Han Lu , Tang Tian , Zhong Zi-Xing , Fang Liang-Xing , Ni Wei-Na , Wang Min-Ge , Wang Xi-Ran , Liu Ya-Hong , Liao Xiao-Ping , Sun Jian TITLE=A Four-Hour Carbapenem Inactivation Method (CIMB.S) Using Bacillus stearothermophilus as Indicator Strain JOURNAL=Frontiers in Medicine VOLUME=7 YEAR=2020 URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2020.00364 DOI=10.3389/fmed.2020.00364 ISSN=2296-858X ABSTRACT=

Objectives: There is an urgent need for accurate and fast diagnostic tests to identify carbapenemase-producing bacteria. Here we used Bacillus stearothermophilus as an indicator strain in the format of the carbapenem inactivation method (CIM) procedure to develop a rapid carbapenemase phenotype detection method: CIM^B.S.

Methods: The CIM^B.S test was derived from the mCIM, where B. stearothermophilus replaced Escherichia coli as the indicator strain. The test bacteria were incubated in the presence of imipenem for 30 min, and then, aliquots were placed on colorimetric plates, and incubation was continued for 3.5 h at 60°C. We examined 134 clinical strains to evaluate the CIM^B.S performance.

Results: The CIM^B.S can be completed in 4 h, and we successfully identified 38/39 (97.4%) carbapenemase-producing Enterobacteriaceae, including 17/18 (94.4%) carbapenemase-producing Pseudomonas aeruginosa and 18/19 (94.7%) carbapenemase-producing Acinetobacter baumannii. All non-carbapenemase producers we tested were negative and included Enterobacteriaceae (n = 36), P. aeruginosa (n = 17), and A. baumannii (n = 5).

Conclusions: The CIM^B.S test is a rapid carbapenemase phenotype detection method requiring only 4 h of total work time and displays high sensitivity and specificity.