AUTHOR=Garth Jaleesa , Easter Molly , Skylar Harris Elex , Sailland Juliette , Kuenzi Lisa , Chung Samuel , Dennis John S. , Baumlin Nathalie , Adewale Adegboyega T. , Rowe Steven M. , King Gwendalyn , Faul Christian , Barnes Jarrod W. , Salathe Matthias , Krick Stefanie TITLE=The Effects of the Anti-aging Protein Klotho on Mucociliary Clearance JOURNAL=Frontiers in Medicine VOLUME=6 YEAR=2020 URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2019.00339 DOI=10.3389/fmed.2019.00339 ISSN=2296-858X ABSTRACT=
α-klotho (KL) is an anti-aging protein and has been shown to exert anti-inflammatory and anti-oxidative effects in the lung and pulmonary diseases such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis. The current study investigated the direct effect of KL on the bronchial epithelium in regards to mucociliary clearance parameters. Primary human bronchial and murine tracheal epithelial cells, cultured, and differentiated at the air liquid interface (ALI), were treated with recombinant KL or infected with a lentiviral vector expressing KL. Airway surface liquid (ASL) volume, airway ion channel activities, and expression levels were analyzed. These experiments were paired with