AUTHOR=Sciascia Savino , Cecchi Irene , Radin Massimo , Rubini Elena , Suárez Ana , Roccatello Dario , Rodríguez-Carrio Javier 

TITLE=IgG Anti-high-Density Lipoproteins Antibodies Discriminate Between Arterial and Venous Events in Thrombotic Antiphospholipid Syndrome Patients

JOURNAL=Frontiers in Medicine

VOLUME=6

YEAR=2019

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2019.00211

DOI=10.3389/fmed.2019.00211

ISSN=2296-858X

ABSTRACT=<p><bold>Introduction:</bold> Recurrent thrombotic events are a hallmark of Antiphospholipid Syndrome (APS). However, biomarkers to identify if a patient with antiphospholipid antibodies (aPL) is at higher risk to develop an arterial or a venous event are lacking. Recently, the pathogenic role of anti-high-density lipoproteins antibodies (anti-HDL) in the occurrence of cardiovascular disease (CVD) in autoimmunity has emerged. The aim of the present study was to evaluate the presence of IgG anti-HDL antibodies in a cohort of thrombotic APS patients and to investigate their association with clinical outcomes.</p><p><bold>Methods:</bold> Serum levels of IgG anti-HDL antibodies, total IgG, and complete aPL profile were assessed in 60 APS patients and 80 healthy donors (HDs) by immunoassays.</p><p><bold>Results:</bold> Higher levels of IgG anti-HDL were found in APS patients compared to HDs (<italic>p</italic> &lt; 0.001), even after correcting for total IgG levels (<italic>p</italic> &lt; 0.001). No associations with treatments or traditional cardiovascular risk factors, except for smoking habit (<italic>p</italic> &lt; 0.0001), were found. Patients who experienced at least one arterial event (<italic>n</italic> = 30) had significantly higher levels of anti-HDL antibodies when compared to patients with venous thrombosis (<italic>n</italic> = 30, <italic>p</italic> = 0.046), this difference being stronger when adjusting for total IgG (<italic>p</italic> = 0.007). Additionally, patients tested positive for antiphosphatidylserine/prothrombin (IgG/IgM) antibodies had significantly higher levels of anti-HDL antibodies (<italic>p</italic> = 0.045).</p><p><bold>Conclusions:</bold> Increased levels of IgG anti-HDL antibodies can be found in APS, mainly in patients with arterial thrombosis, independently of aPL antibodies and traditional risk factors. These findings point to a role of anti-HDL antibodies in APS and support their use as a potential biomarker for arterial thrombotic events.</p>