AUTHOR=Kolb Manon , Lazarevic Vladimir , Emonet Stéphane , Calmy Alexandra , Girard Myriam , Gaïa Nadia , Charretier Yannick , Cherkaoui Abdessalam , Keller Peter , Huber Christoph , Schrenzel Jacques TITLE=Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis JOURNAL=Frontiers in Medicine VOLUME=6 YEAR=2019 URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2019.00203 DOI=10.3389/fmed.2019.00203 ISSN=2296-858X ABSTRACT=

Diagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing (NGS) is an emerging approach that allows pathogen identification in challenging situations, as evidenced by a clinical case. We sequenced the DNA extracted from the surgically-removed frozen valve tissue from a patient with suspected infective endocarditis with negative blood and valve cultures. Mapping of the sequence reads against reference genomic sequences, a 16S rRNA gene database and clade-specific marker genes suggested an infection caused by Cardiobacterium hominis.