AUTHOR=Molina-Vila Miguel A. , Mayo-de-las-Casas Clara , Giménez-Capitán Ana , Jordana-Ariza Núria , Garzón Mónica , Balada Ariadna , Villatoro Sergi , Teixidó Cristina , García-Peláez Beatriz , Aguado Cristina , Catalán María José , Campos Raquel , Pérez-Rosado Ana , Bertran-Alamillo Jordi , Martínez-Bueno Alejandro , Gil María-de-los-Llanos , González-Cao María , González Xavier , Morales-Espinosa Daniela , Viteri Santiago , Karachaliou Niki , Rosell Rafael TITLE=Liquid Biopsy in Non-Small Cell Lung Cancer JOURNAL=Frontiers in Medicine VOLUME=3 YEAR=2016 URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2016.00069 DOI=10.3389/fmed.2016.00069 ISSN=2296-858X ABSTRACT=

Liquid biopsy analyses are already incorporated in the routine clinical practice in many hospitals and oncology departments worldwide, improving the selection of treatments and monitoring of lung cancer patients. Although they have not yet reached its full potential, liquid biopsy-based tests will soon be as widespread as “standard” biopsies and imaging techniques, offering invaluable diagnostic, prognostic, and predictive information. This review summarizes the techniques available for the isolation and analysis of circulating free DNA and RNA, exosomes, tumor-educated platelets, and circulating tumor cells from the blood of cancer patients, presents the methodological challenges associated with each of these materials, and discusses the clinical applications of liquid biopsy testing in lung cancer.