AUTHOR=Booth Valerie TITLE=Deuterium Solid State NMR Studies of Intact Bacteria Treated With Antimicrobial Peptides JOURNAL=Frontiers in Medical Technology VOLUME=2 YEAR=2021 URL=https://www.frontiersin.org/journals/medical-technology/articles/10.3389/fmedt.2020.621572 DOI=10.3389/fmedt.2020.621572 ISSN=2673-3129 ABSTRACT=

Solid state NMR has been tremendously useful in characterizing the structure and dynamics of model membranes composed of simple lipid mixtures. Model lipid studies employing solid state NMR have included important work revealing how membrane bilayer structure and dynamics are affected by molecules such as antimicrobial peptides (AMPs). However, solid state NMR need not be applied only to model membranes, but can also be used with living, intact cells. NMR of whole cells holds promise for helping resolve some unsolved mysteries about how bacteria interact with AMPs. This mini-review will focus on recent studies using 2H NMR to study how treatment with AMPs affect membranes in intact bacteria.