AUTHOR=Benfield Aurélie H. , Henriques Sónia Troeira 

TITLE=Mode-of-Action of Antimicrobial Peptides: Membrane Disruption vs. Intracellular Mechanisms

JOURNAL=Frontiers in Medical Technology

VOLUME=2

YEAR=2020

URL=https://www.frontiersin.org/journals/medical-technology/articles/10.3389/fmedt.2020.610997

DOI=10.3389/fmedt.2020.610997

ISSN=2673-3129

ABSTRACT=<p>Antimicrobial peptides are an attractive alternative to traditional antibiotics, due to their physicochemical properties, activity toward a broad spectrum of bacteria, and mode-of-actions distinct from those used by current antibiotics. In general, antimicrobial peptides kill bacteria by either disrupting their membrane, or by entering inside bacterial cells to interact with intracellular components. Characterization of their mode-of-action is essential to improve their activity, avoid resistance in bacterial pathogens, and accelerate their use as therapeutics. Here we review experimental biophysical tools that can be employed with model membranes and bacterial cells to characterize the mode-of-action of antimicrobial peptides.</p>