Evaluation of multimodal imaging guided multifunctional bio-targeted reactive oxygen species production synergists: a preliminary study

Ou xia¹Tang Yu²Yang haiyan^3*Du Yan²Zhang zhong²Lin li²Wang qi²Zou Jianzhong^2*

• ¹Chongqing General Hospital ,Chongqing University, State Key Laboratory of Ultrasound in Medicine and Engineering, Chongqing Medical University, Chongqing, China
• ²State Key Laboratory of Ultrasound in Medicine and Engineering, Chongqing Medical University, Chongqing, China
• ³Chongqing General Hospital, Chongqing University, State Key Laboratory of Ultrasound in Medicine and Engineering, Chongqing Medical University, Chongqing, China

Pulse focused ultrasound (PFUS) is a novel non-invasive technique with reduced risk of thermal damage to normal tissue surrounding. As a result of the rapid attenuation of ultrasonic energy, synergists are critical to therapy process. However, due to various factors and the complex microenvironment in the tumor, synergists existed have limitations such as poor targeting, incomplete imaging during therapy, and tumor residue after therapy. Given that, the study aims to construct bio-targeted reactive oxygen species (ROS)-producing synergists consisting of Bifidobacterium bifidum (B. bifidum) that actively homing to the tumor and multifunctional ROS producing nanoparticles loaded with chlorin e6(Ce6) and perfuorohexane (PFH). The synergists are expected to achieve targeted and synergistic PFUS ablation, as well as multimodal imaging to guide diagnosis and therapy. The Ce6 carried in it is precisely released during PFUS and produces ROS, realizing PFUS combined with sonodynamic therapy(SDT) anti-tumor treatment. The strategy is expected to make up for the shortcomings of existing PFUS collaborative therapy, and provide an idea for tumor diagnosis and treatment.