AUTHOR=McNamara Marilyn C. , Niaraki-Asli Amir Ehsan , Guo Jingshuai , Okuzono Jasmin , Montazami Reza , Hashemi Nicole N. TITLE=Enhancing the Conductivity of Cell-Laden Alginate Microfibers With Aqueous Graphene for Neural Applications JOURNAL=Frontiers in Materials VOLUME=7 YEAR=2020 URL=https://www.frontiersin.org/journals/materials/articles/10.3389/fmats.2020.00061 DOI=10.3389/fmats.2020.00061 ISSN=2296-8016 ABSTRACT=

Microfluidically manufacturing graphene-alginate microfibers create possibilities for encapsulating rat neural cells within conductive 3D tissue scaffolding to enable the creation of real-time 3D sensing arrays with high physiological relavancy. Cells are encapsulated using the biopolymer alginate, which is combined with graphene to create a cell-containing hydrogel with increased electrical conductivity. Resulting novel alginate-graphene microfibers showed a 2.5-fold increase over pure alginate microfibers, but did not show significant differences in size and porosity. Cells encapsulated within the microfibers survive for up to 8 days, and maintain ~20% live cells over that duration. The biocompatible aqueous graphene suspension used in this investigation was obtained via liquid phase exfoliation of pristine graphite, to create a graphene-alginate pre-hydrogel solution.