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ORIGINAL RESEARCH article

Front. Mar. Sci.
Sec. Marine Fisheries, Aquaculture and Living Resources
Volume 11 - 2024 | doi: 10.3389/fmars.2024.1495938
This article is part of the Research Topic Innovative Approaches to Modulate Fish Gut Microbiota for Disease Management in Aquaculture View all articles

Effects of Oyster Shell Addition on Shrimp Aquaculture and the Dynamic Succession of Surface Biofilm Microbial Communities

Provisionally accepted
Huang Xianke Huang Xianke 1,2,3Xiao Guoqiang Xiao Guoqiang 1,2,3*Zhang Xiang Zhang Xiang 1,2,3*Teng Shuangshuang Teng Shuangshuang 1,2,3*Li Ming Li Ming 1,2,3*Cai Yilong Cai Yilong 1,2,3*Chen Ran Chen Ran 1,2,3*Huang Xiaolin Huang Xiaolin 1,2,3*
  • 1 Zhejiang Mariculture Research Institute, Wenzhou, China
  • 2 Wenzhou key Laboratory of Marine Biological Genetics and Breeding, WenZhou, China
  • 3 Zhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, WenZhou, China

The final, formatted version of the article will be published soon.

    In the context of Litopenaeus vannamei aquaculture, the incorporation of oyster shells has proven beneficial for enhancing water quality and the growth conditions of the shrimp. Nonetheless, the specific effects of in-situ water treatment using oyster shells on water quality and shrimp growth, along with the composition and succession dynamics of the microbial community within oyster shell biofilms, have yet to be thoroughly investigated. This study established control, low-concentration, and high-concentration oyster shell addition groups to emulate the in-situ water treatment environment with oyster shells, with the objective of elucidating the impacts of oyster shell addition on the aquaculture setting. The results showed that the addition of oyster shells could significantly improve the length (F = 12.248 , P = 0.005), weight(F = 138.234 , P < 0.001), and survival rate (F = 15.248 , P < 0.001) of shrimp, while there were no significant differences in the length (F = -1.233 , P = 0.267) and survival rate (F = -2.143 , P = 0.076) between the high and low concentration groups. Additionally, oyster shell addition resulted in elevated phosphate levels (F = 74.92, P < 0.001 in Day 70), diminished nitrite levels (F = 5.276, P = 0.031 in Day 56), and increased nitrate concentrations (F = 9.421, P = 0.006 in Day 70). Within the biofilms, the relative abundances of Ruegeria, Tenacibaculum, BD2-11_terrestrial_group, and Kapabacteriales exhibited significant declines over time, whereas the relative abundance of Nitrospira demonstrated a marked increase, ultimately emerging as the predominant bacterium (Relative abundance 31.8%) in the biofilms during the latter stages of the experiment. Nitrospira also exhibited a notably higher relative abundance in the microbial community of the experimental water group relative to the control group (F = 2.265, P = 0.001). This research offers valuable insights for the application of oyster shells in shrimp farming and contributes to the theoretical underpinnings necessary for advancing our understanding of the mechanisms through which oyster shell biofilms enhance water quality and foster shrimp health.

    Keywords: Litopenaeus vannamei, Oyster shells, Biofilm, microbial community, Water Quality

    Received: 13 Sep 2024; Accepted: 08 Oct 2024.

    Copyright: © 2024 Xianke, Guoqiang, Xiang, Shuangshuang, Ming, Yilong, Ran and Xiaolin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Xiao Guoqiang, Zhejiang Mariculture Research Institute, Wenzhou, China
    Zhang Xiang, Wenzhou key Laboratory of Marine Biological Genetics and Breeding, WenZhou, China
    Teng Shuangshuang, Wenzhou key Laboratory of Marine Biological Genetics and Breeding, WenZhou, China
    Li Ming, Wenzhou key Laboratory of Marine Biological Genetics and Breeding, WenZhou, China
    Cai Yilong, Wenzhou key Laboratory of Marine Biological Genetics and Breeding, WenZhou, China
    Chen Ran, Wenzhou key Laboratory of Marine Biological Genetics and Breeding, WenZhou, China
    Huang Xiaolin, Wenzhou key Laboratory of Marine Biological Genetics and Breeding, WenZhou, China

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