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ORIGINAL RESEARCH article
Front. Mar. Sci.
Sec. Global Change and the Future Ocean
Volume 11 - 2024 |
doi: 10.3389/fmars.2024.1492142
Pseudoalteromonas flavipulchra as a dual-functional probiotic for aquaculture: Enhancing microalgae growth and antagonizing Vibrio pathogens
Provisionally accepted- Ningbo University, Ningbo, China
Vibrio disease is a prevalent bacterial infection in aquaculture, and using bacteria with antagonistic properties against Vibrio species as probiotics has emerged as a promising method for disease prevention. Additionally, low biomass productivity of microalgae feed remains a significant bottleneck in bivalve aquaculture. Therefore, it is essential to screen for bacteria that both enhance microalgae growth and inhibit Vibrio pathogens. In this work, we screened seven microalgae growth-promoting bacterial for their antibacterial activity against three Vibrio pathogens, i.e., Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio cholerae, and found that Pseudoalteromonas flavipulchra exhibited antagonistic effects against all three tested Vibrio species. The extracellular products (ECP) of P. flavipulchra exhibited stable antibacterial activity, but it was lost after treatment with heat, alkali, or proteinase K. The proteinaceous material isolated from the ECP by precipitation with 90% saturated ammonium sulfate exhibited antibacterial activity in a concentration-dependent manner. According to these findings P. flavipulchra could be a promising dual-functional probiotic for aquaculture.
Keywords: Microalgae growth-promoting bacteria, Pseudoalteromonas flavipulchra, Vibrio, Antibacterial activity, Antibacterial substance
Received: 06 Sep 2024; Accepted: 21 Oct 2024.
Copyright: © 2024 Wu, Xu, Shao, Wang, Cao and Xu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jiayi Cao, Ningbo University, Ningbo, China
Jilin Xu, Ningbo University, Ningbo, China
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