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ORIGINAL RESEARCH article
Front. Mar. Sci.
Sec. Aquatic Physiology
Volume 11 - 2024 |
doi: 10.3389/fmars.2024.1462905
This article is part of the Research Topic Physiological Response and Molecular Mechanism of Hypoxic Response in Fish View all articles
Transcriptomic Responses to Hypoxia in two Populations of Eastern Oyster with Differing Tolerance
Provisionally accepted- 1 Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, United States
- 2 School of Animal Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA, United States
The eastern oyster, Crassostrea virginica, is a keystone species native to the Gulf of Mexico and Atlantic coasts of the United States and Canada. It provides habitat for other marine organisms and makes up the majority of oyster production in the United States. Despite its tolerance to hypoxic conditions, C. virginica is threatened by anthropogenic climate change, which is increasing both average temperature and the frequency and severity of hypoxic events. In this study, we explore the differences in hypoxia-transcriptional response between two populations of eastern oysters with known differences in hypoxia tolerance at three time points over the course of a 5-day hypoxia treatment. We identified sets of genes involved in the hypoxia response and found differences in both the timing and baseline expression of hypoxia-responsive genes between tolerant and sensitive populations, consistent with a scenario of local adaptation. Analysis of differential gene expression between the two populations and conditions revealed two gene modules with higher baseline expression of hypoxia-sensitive genes in the more hypoxia tolerant population. Key GO terms for genes corresponding to differences between populations include DNA repair, ribosome biogenesis, and ribonucleotide binding. Our results imply that differences in hypoxia tolerance between populations could be due to genetic frontloading of hypoxia response pathways in the more tolerant population.
Keywords: Hypoxia1, Climate change2, Crassostrea virginica3, Transcriptomics4, gene expression5
Received: 10 Jul 2024; Accepted: 03 Sep 2024.
Copyright: © 2024 Crable, Rodriguez, Vaidya, Coxe, La Peyre and Kelly. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Emma L. Crable, Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, United States
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