Skip to main content

ORIGINAL RESEARCH article

Front. Mar. Sci.
Sec. Marine Fisheries, Aquaculture and Living Resources
Volume 11 - 2024 | doi: 10.3389/fmars.2024.1446927

Comparison of the effects of host-associated (autochthonous) and commercial probiotics on immune responses, growth parameters and intestinal microbiota of Caspian whitefish (Rutilus frisii kutum) fry

Provisionally accepted
Seyed Hossein Hoseinifar Seyed Hossein Hoseinifar 1*Seyyed Morteza Hoseini Seyyed Morteza Hoseini 2Ali Taheri Mirghaed Ali Taheri Mirghaed 3Melika Ghelichpour Melika Ghelichpour 3Hesamaddin S. Aski Hesamaddin S. Aski 4Hien V. Doan Hien V. Doan 5Ehab El-Haroun Ehab El-Haroun 6Roghieh Safari Roghieh Safari 1Majid Khanzadeh Majid Khanzadeh 1,7
  • 1 Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
  • 2 Iranian Fisheries Science Research Institute (IFSRI), Gorgan, Golestan, Iran
  • 3 Department of Aquatic Health and Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Alborz, Iran
  • 4 Golestan University of Medical Sciences, Gorgan, Golestan, Iran
  • 5 Department of Animal Science and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
  • 6 Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Giza, Egypt
  • 7 Academic Center for Education, Culture and Research, Tehran, Tehran, Iran

The final, formatted version of the article will be published soon.

    This study investigates the effects of host-associated probiotic (HAP) vs commercial probiotic (CP) on the growth performance, antioxidant defence and immunity of Caspian whitefish fry. Three hundred whitefish fry (1.15 ± 0.03 g) were randomly divided into five treatments in triplicate. Treatments included feeding with the control group (zero), P. acidilactici as a commercial probiotic (CP) at 6 × 10 8 CFU g -1 , and HA Pediococcus pentosaceus at 10 6 , 10 7 and 10 8 CFU g -1 for eight weeks. Dietary HAP and CP did not have significant effects on growth indices compared to the control group (P > 0.05). However, HAP at 10 7 and 10 8 CFU g -1 and CP significantly increased protein in whitefish carcasses compared to the control group (P < 0.05).Different levels of HAP and CP had a significant effect on whole-body extract (WBE) lysozyme (LZM) activity (P < 0.05). HAP treatment significantly increased WBE ACH50 and bactericidal activity compared to the control and CP group (P < 0.05). Also, in the case of mucosal immune response, different levels of HAP could significantly increase LZM, total immunoglobulin (Ig),agglutination titer, protease and alkaline phosphatase activity compared to the control group (P < 0.05). Whitefish fed HAP showed a significant increase in the activity of WBE antioxidant parameters (SOD, CAT and GPx) compared to the control group (P < 0.05). Also, feeding withHAP could significantly increase autochthonous LAB levels compared control group (P < 0.05); while the total count of intestinal heterotrophic bacteria was not affected (P > 0.05). Overall, the present study showed HA Pediococcus pentosaceus can be considered as beneficial feed additive for whitefish.

    Keywords: Whitefish, autochthonous probiotic, Pediococcus pentosaceus, Growth indicators, ٤٣ Nonspecific immunity ٤٤ ٤٥

    Received: 14 Jun 2024; Accepted: 01 Aug 2024.

    Copyright: © 2024 Hoseinifar, Hoseini, Taheri Mirghaed, Ghelichpour, Aski, Doan, El-Haroun, Safari and Khanzadeh. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Seyed Hossein Hoseinifar, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.