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ORIGINAL RESEARCH article

Front. Mar. Sci.
Sec. Marine Biotechnology and Bioproducts
Volume 11 - 2024 | doi: 10.3389/fmars.2024.1444618

Synthesis and characterization of Turbinaria ornata mediated Zn/ZnO green nanoparticles as potential antioxidant and anti-diabetic agent of enhanced activity

Provisionally accepted
Amira S. Diab Amira S. Diab 1Khaled N. Elsayed Khaled N. Elsayed 1Ahmed M. El-Sherbeeny Ahmed M. El-Sherbeeny 2Wail Al Zoubi Wail Al Zoubi 3Mostafa Abukhadra Mostafa Abukhadra 1*
  • 1 Beni-Suef University, Beni-Suef, Egypt
  • 2 King Saud University, Riyadh, Riyadh, Saudi Arabia
  • 3 Yeungnam University, Gyeongsan, North Gyeongsang, Republic of Korea

The final, formatted version of the article will be published soon.

    Turbinaria ornata marine macro-algae (TUN) have been applied as carriers for the metallic zinc/ZnO blended nanoparticles, which were synthesized by implementing the extracted phytochemicals of the algae.The resulting hybrid bio-composite (Zn@ZnO/TUN) was characterized as a potential product of promising antioxidant and antidiabetic characteristics in synergetic studies. The obtained composite demonstrate t6he existing or complex biological active groups related to zinc (Zn-O stretching and tetrahedral Zn coordination) and organic groups (amino, methyl, carboxylic, alkynes, P=O, C-C-O, C=N, and N-O) corresponding to the extracted phytochemicals of algae (polysaccharides, phospholipids, lipids, fucose, and phosphodiester). The assessment of Zn@ZnO/TUN hybrid as an anti-oxidant agent validated excellent effectiveness towards the commonly examined radicals (DPPH (88.2 ± 1.44%), nitric oxide (92.7 ± 1.71%), ABTS (90.5 ± 1.8%), and O 2•-(30.6 ± 1.32%), considering the determined performance for the commercially used standard (ascorbic acid). Regarding the antidiabetic properties, the incorporation of Zn@ZnO/TUN inhibits the function and activities of the key oxidizing enzymes, either the commercial forms (α-amylase (88.7 ± 1.3%), α-glucosidase (98.4 ± 1.3%), and amyloglucosidase (97.3 ± 1.4%) or the crude intestinal active forms (α-amylase (66.2 ± 1.4%) and α-glucosidase (95.1 ± 1.5%). This inhibitory effectiveness of Zn@ZnO/TUN is significantly better than the measured performances using commercialized miglitol drugs and slightly better than acarbose.Considering the expense and adverse effects of conventional medications, the synthesized Zn@ZnO/TUN blend could be evaluated as a marketable antidiabetic and antioxidant medication. The findings also demonstrate the influence of the derived phytochemicals from Turbinaria ornata and the incorporation of its algae residuals as carriers for the metal nanoparticles on the biological function of the composite. The cytotoxicity investigation reflected safety effect of the composite on colorectal fibroblast cells (CCD-18Co) (96.3% cell viability) and inhibition effect on cancerous colorectal cells (HCT-116) (47.3 % cell viability)

    Keywords: Turbinaria ornata, Green synthesis, Zinc, composite, antioxidant, antidiabetic

    Received: 06 Jun 2024; Accepted: 19 Jul 2024.

    Copyright: © 2024 Diab, Elsayed, El-Sherbeeny, Al Zoubi and Abukhadra. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Mostafa Abukhadra, Beni-Suef University, Beni-Suef, Egypt

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