The final, formatted version of the article will be published soon.
BRIEF RESEARCH REPORT article
Front. Mar. Sci.
Sec. Aquatic Physiology
Volume 11 - 2024 |
doi: 10.3389/fmars.2024.1443863
Transcription profiling reveals co-regulation mechanism of gene expression related to growth and mineralization induced by pearl cultivation in Hyriopsis cumingii
Provisionally accepted
Yige Chen
1
Yuanbin Yao
1*
Xiaoya Shen
1
Yuanshuai Fu
1
Jiale LI
1
Zhiyi Bai
1*
Xiangli Bian
2*
Wenjuan Li
1*
Shijun Liu
3*- 1 Shanghai Ocean University, Shanghai, China
- 2 Department of Pediatrics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China
- 3 Shanghai Mugao Biotechnology Co., Lt'd, Shanghai, China
Hyriopsis cumingii is a major freshwater pearl mussel in the world. In order to investigate the functional roles of the growth and mineralization-related genes involved in the pearl formation process after the inserting nucleus into the mantle tissue of Hyriopsis cumingii, we conducted a transcriptome analysis of data from different time periods after the pearl-nucleus insertion. We screened a total of 1898 growth-related unigenes and 716 mineralization-related unigenes, including 12 growth-related differential genes and 8 mineralization-related differential genes that showed sustained differential expression throughout the pearl formation. And the results of the gene expression patterns among samples at different time points showed that the Mcon group and the M05d group clustered together, the M50d group and the M90d group clustered together, while M20d group clustered separately. The overall density distribution of differential gene analysis at different periods after pearl-nucleus insertion of Hyriopsis cumingii, cluster analysis, differential gene analysis, differential gene differential and co-expression analysis together revealed that growth and mineralization-related genes have the same expression pattern and jointly regulate pearl formation. GO and KEGG enrichment revealed that the same functional cluster and pathway were together present on the enrichment results of both growth and mineralization-related genes, suggesting a synergistic effect between growth and mineralization. We found that genes such as bone morphogenetic proteins (BMPs) and calmodulin (CALM) are jointly involved in growth and mineralization processes. The study indicate that growth genes cooperate with mineralization genes to play biological roles in the early process of pearl formation in Hyriopsis cumingii.
Keywords: Hyriopsis cumingii, pearl-nucleus insertion, growth and mineralization, co-regulation, Transcriptome
Received: 14 Jun 2024; Accepted: 22 Jul 2024.
Copyright: © 2024 Chen, Yao, Shen, Fu, LI, Bai, Bian, Li and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Yuanbin Yao, Shanghai Ocean University, Shanghai, China
Zhiyi Bai, Shanghai Ocean University, Shanghai, China
Xiangli Bian, Department of Pediatrics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China
Wenjuan Li, Shanghai Ocean University, Shanghai, China
Shijun Liu, Shanghai Mugao Biotechnology Co., Lt'd, Shanghai, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.