Franciele R. De Castro ^1,2* Danielle V. Harris ^3* Susannah J. Buchan ^1,4,5 Naysa Balcazar ⁶ Brian S. Miller ⁷

• ¹ Center for Oceanographic Research COPAS COASTAL, Universidad de Concepción, Concepción, Región del Bio, Chile
• ² Aqualie Institute, Minas Gerais, Brazil
• ³ Centre for Research into Ecological and Environmental Modelling, University of St Andrews, St Andrews, United Kingdom
• ⁴ Department of Oceanography, Faculty of Natural and Oceanographic Sciences, University of Concepción, Concepción, VIII Biobío Region, Chile
• ⁵ Centro de Estudios Avanzados en Zonas Áridas (CEAZA), La Serena, Chile
• ⁶ Other, Queensland, Australia
• ⁷ Australian Antarctic Division, Hobart, Tasmania, Australia

We explore the utility of estimating the density of calls of baleen whales for better understanding acoustic trends over time. We consider as a case study stereotyped 'song' calls of Antarctic blue whales (Balaenoptera musculus intermedia) on their Antarctic feeding grounds over the course of a year-long, continuous recording from 2014. The recording was made in the Southern Ocean from a deep-water autonomous hydrophone moored near the seafloor in the Eastern Indian sector of the Antarctic. We estimated call density seasonally via a Monte-Carlo simulation based on the passive sonar equation, and compared our estimates to seasonal estimates of detection rate, which are commonly reported in acoustic studies of Antarctic blue whales. The resulting seasonal call densities at our Antarctic site were strongly influenced by seasonally varying noise levels, which in turn yielded seasonal differences in detection range. Incorporating the seasonal estimates of detection area into our analysis revealed a pattern of call densities in accord with historic (non-acoustic) knowledge of Antarctic blue whale seasonal distribution and migrations, a pattern that differed from seasonal detection rates. Furthermore, our methods for estimating call densities produced results that were more statistically robust for comparison across sites and time and more meaningful for interpretation of biological trends compared to detection rates alone. These advantages came at the cost of a more complex analysis that accounts for the large variability in detection range of different sounds that occur in Antarctic waters, and also accounts for the performance and biases introduced by automated algorithms to detect sounds. Despite the additional analytical complexities, broader usage of call densities, instead of detection rates, has the potential to yield a standardized, statistically robust, biologically informative, global investigation of acoustic trends in baleen whale sounds recorded on single hydrophones, especially in the remote and difficult to access Antarctic.