AUTHOR=Yao Ting-Yan , Miao Qing-Tao , Sun Xuan-Yang , Rui Chen , Xie Qing-Ping , Wang Xu-Bo 

TITLE=The identification of toll-like receptor genes in large yellow croaker (Larimichthys crocea): provides insights into its environmental adaptation to biological and abiotic stresses

JOURNAL=Frontiers in Marine Science

VOLUME=11

YEAR=2024

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2024.1404183

DOI=10.3389/fmars.2024.1404183

ISSN=2296-7745

ABSTRACT=<p>Large yellow croaker (<italic>Larimichthys crocea</italic>), the most developing fish of China, suffering from both biotic and abiotic stressors. A genome-wide study was performed for the first time to investigate the roles of Toll-like receptor genes in large yellow croaker (<italic>LcTLRs</italic>) working in hypoxia response and <italic>Aeromonas hydrophila</italic> infection. 12 <italic>TLR</italic> genes were identified and annotated, and phylogenetic tree, analysis of structure and motifs demonstrated that <italic>TLRs</italic> were highly conserved. To further investigate the <italic>LcTLRs</italic> under environmental stress, expression pattern analysis of hypoxic response and <italic>Aeromonas hydrophila</italic> infection revealed that there were nine and six members of <italic>LcTLRs</italic> showing significant differential expression, indicating that they were concerned in hypoxia stress and disease responses. Meanwhile, their expression levels were validated utilizing qPCR. Taken together, a greater appreciation and understanding of <italic>TLRs</italic> function in the reaction to biotic and abiotic stress would ultimately lead to more efficiently environmental adaptation in large yellow croaker.</p>