AUTHOR=Ma Xiaoxue , Gu Wenfei , Yang Chuanyan , He Zhaoyu , Fan Hongmei , Wang Lingling , Song Linsheng 

TITLE=Inhibition of TRPA1-like alleviated unfolded protein response and apoptosis by regulating cytoplasmic Ca2+ in Yesso scallop Patinopecten yessoensis under high temperature stress

JOURNAL=Frontiers in Marine Science

VOLUME=11

YEAR=2024

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2024.1388382

DOI=10.3389/fmars.2024.1388382

ISSN=2296-7745

ABSTRACT=<p>Transient receptor potential ankyrin subtype 1 (TRPA1) is a nonselective cation channel protein typically forms ion channels that regulate intracellular calcium homeostasis, and can be induced by temperature and various chemicals. In the present study, the involvement of <italic>Py</italic>TRPA1-like in regulating unfolded protein response (UPR) and apoptosis in Yesso Scallop <italic>Patinopecten yessoen</italic>sis under high temperature stress was investigated. The mRNA transcripts of <italic>Py</italic>TRPA1-like were detected in haemocytes and all the examined tissues with the highest expression level in mantle. After TRPA1 activator (allyl-isothiocyanate, AITC) and high temperature (25°C) treatment, the expression level of <italic>Py</italic>TRPA1-like mRNA and the Ca<sup>2+</sup> content in haemocytes increased significantly (<italic>p</italic> &lt; 0.05) at 3 h, and then recovered to the normal level at 12 h, and the expression level of <italic>Py</italic>GRP78, <italic>Py</italic>IRE1, <italic>Py</italic>ATF6β, <italic>Py</italic>PERK and <italic>Py</italic>Caspase-3 mRNA in haemocytes, and Caspase-3 activity and apoptosis rate were also significantly upregulated (<italic>p</italic> &lt; 0.05). After TRPA1 antagonist (HC-030031) and high temperature (25°C) treatment, the intracellular Ca<sup>2+</sup> content, the transcripts of <italic>Py</italic>GRP78, <italic>Py</italic>IRE1 and <italic>Py</italic>Caspase-3 in haemocytes, as well as the Caspase-3 activity and apoptosis rate decreased significantly compared to the control group (<italic>p</italic> &lt; 0.05), while the Ca<sup>2+</sup> distribution in haemocytes showed no difference with that in control group. These results collectively suggest that <italic>Py</italic>TRPA1-like plays important roles in regulating UPR and apoptosis by mediating calcium influx under high temperature stress in scallop <italic>P. yessoensis</italic>.</p>