AUTHOR=Virués-Segovia Jorge R. , Pinedo Cristina , Zorrilla David , Sánchez-Márquez Jesús , Sánchez Pilar , Ramos María C. , de la Cruz Mercedes , Aleu Josefina , Durán-Patrón Rosa 

TITLE=Discovery of new eremophilanes from the marine-derived fungus Emericellopsis maritima BC17 by culture conditions changes: evaluation of cytotoxic and antimicrobial activities

JOURNAL=Frontiers in Marine Science

VOLUME=11

YEAR=2024

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2024.1386175

DOI=10.3389/fmars.2024.1386175

ISSN=2296-7745

ABSTRACT=<p>In our previous studies, the marine-derived fungus <italic>Emericellopsis maritima</italic> BC17 was found to produce new eremophilane-type sesquiterpenoids on solid media. In order to explore its potential to produce more metabolites, <italic>E. maritima</italic> BC17 was subjected to a one strain-many compounds (OSMAC) analysis leading to the discovery of three new eremophilanes (<bold>1</bold>-<bold>3</bold>) and fourteen known derivatives (<bold>4</bold>-<bold>17</bold>) in the liquid media Czapek Dox and PDB. Their structures were established by extensive analyses of the 1D and 2D NMR, and HRESIMS data, as well as ECD data for the assignment of their absolute configurations. Antitumoral and antimicrobial activities of the isolated metabolites <bold>1</bold>, <bold>3</bold>, <bold>11</bold>, and <bold>15</bold> were investigated. PR toxin 3-deacetyl (<bold>15</bold>) exhibited cytotoxic activity against HepG2, MCF-7, A549, A2058 and Mia PaCa-2 human cancer cell lines with IC<sub>50</sub> values ranging from 2.5 to 14.7 µM. In addition, <bold>15</bold> exhibited selective activity against methicillin-sensitive <italic>Staphylococcus aureus</italic> ATCC29213 at the highest concentration tested of 128 µg/mL.</p>