AUTHOR=Jia Shaoting , Jin Ling , Lv Jianjian , Wang Jiajia , Li Jitao , Liu Ping , Li Jian 

TITLE=Comparative transcriptomic analysis and validation of the ovary and testis in the ridgetail white prawn (Exopalaemon carinicauda)

JOURNAL=Frontiers in Marine Science

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2022.995790

DOI=10.3389/fmars.2022.995790

ISSN=2296-7745

ABSTRACT=<p>The ridgetail white prawn, <italic>Exopalaemon carinicauda</italic> is an important cultured seawater species in China. As female <italic>E.carinicauda</italic> grows faster than males, it is significant to search for the differentially expressed genes (DEGs) between males and females. However, there is no public available <italic>E.carinicauda</italic> genome data, and genes related to <italic>E. carinicauda</italic> sex differences are unclear. In this study, the transcriptome sequencing for ovary and testis tissues of <italic>E. carinicauda</italic> were conducted, and 20,891 DEGs were identified including 11,709 up-regulated DEGs and 9,182 downregulated DEGs. The functional categories related to meiosis and reproduction were enriched as well as the steroidogenesis KEGG pathway was clustered. Furthermore, the genes related to male reproduction and cell cycle were dug out which were verified by real-time PCR. In addition, two-color fluorescent <italic>in situ</italic> hybridization result showed that <italic>foxj1b</italic> might play roles during early stage of the ovary development. Therefore, our result provides clues for the study of genes related to reproduction and sex difference in <italic>E.carinicauda</italic>.</p>