AUTHOR=Rojas-Vargas Jorge , Adaya Libertad , Silva-Jiménez Hortencia , Licea-Navarro Alexei Fedorovish , Sanchez-Flores Alejandro , Gracia Adolfo , Pardo-López Liliana 

TITLE=Oil-degrading bacterial consortium from Gulf of Mexico designed by a factorial method, reveals stable population dynamics

JOURNAL=Frontiers in Marine Science

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2022.962071

DOI=10.3389/fmars.2022.962071

ISSN=2296-7745

ABSTRACT=<p>We describe an assembled marine bacterial consortium designed for bioremediation of oil-contaminated seawater, based on a statistical method using a Plackett-Burman (PB) experimental approach. The final consortium consists of four bacteria isolated from the Gulf of Mexico, from four genera: <italic>Pseudomonas, Halopseudomonas, Paenarthrobacter</italic>, and <italic>Alcanivorax</italic>. Individually, bacterial oil removal by these microorganisms was evaluated by gravimetry, reaching 39% at maximum after 75 days, whereas in consortium it was ~62%. We also measured biodegradation levels by Gas Chromatography/Mass Spectrometry (GC-MS) observing 12 polyaromatic hydrocarbons (PAHs) degradation analyzed and <italic>n</italic>-alkanes degradation with a preference for specific chain length. Consortium population analysis using the V3-V4 region of 16S rRNA showed a stable community, suggesting that the metabolic load was distributed among bacteria and that stable dynamic interactions were achieved. In this work, we show that the use of a factorial method for synthetic consortium design offers the possibility of improving oil degradation efficiency with stable bacterial populations.</p>