AUTHOR=Viegas Ivan , Palma Mariana , Plagnes-Juan Elisabeth , Silva Emanuel , Rito João , Henriques Luís F. , Tavares Ludgero C. , Ozório Rodrigo O. A. , Panserat Stéphane , Magnoni Leonardo J. TITLE=On the Utilization of Dietary Glycerol in Carnivorous Fish—Part II: Insights Into Lipid Metabolism of Rainbow Trout (Oncorhynchus mykiss) and European Seabass (Dicentrarchus labrax) JOURNAL=Frontiers in Marine Science VOLUME=9 YEAR=2022 URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2022.836612 DOI=10.3389/fmars.2022.836612 ISSN=2296-7745 ABSTRACT=
Glycerol is the by-product of biodiesel production and its utilisation in fish feed has recently gained relevance. As an important metabolic intermediate and structural component of triacylglycerol (TAG), it is still unclear whether its supplementation affects lipid utilisation and/or deposition in different tissues. Accordingly, a set of studies was aimed to evaluate how increasing levels of dietary glycerol (0, 2.5 and 5%) affect lipid synthesis in the liver, muscle and adipose tissue. After a growth trial with rainbow trout (8 weeks) and European seabass (6 weeks) fish were sampled at 6 and 24 h to assess mRNA levels of lipid metabolism-related enzymes. The remaining fish were subjected to a metabolic trial consisting of a 6-day residence in deuterated water (2H2O) for metabolic flux calculations. This study stands as the second part of a broader experiment that also aimed at evaluating the carbohydrate metabolism (