AUTHOR=Boles Sara E. , Neylan Isabelle P. , Rogers-Bennett Laura , Gross Jackson A. 

TITLE=Evaluation of Gonad Reproductive Condition Using Non-invasive Ultrasonography in Red Abalone (Haliotis rufescens)

JOURNAL=Frontiers in Marine Science

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2022.784481

DOI=10.3389/fmars.2022.784481

ISSN=2296-7745

ABSTRACT=<p>The reproductive status in abalone (<italic>Haliotis</italic> spp.) can be difficult to discern using traditional visual inspection methods and may therefore rely on lethal histological analyses for a definitive determination. With endangered species of abalone, lethal histological examinations are strictly prohibited due to their imperiled status. This research investigated the effectiveness of using non-invasive ultrasound technology as an alternate means of identifying the reproductive status of abalone rather than through invasive biopsies or lethal histological methods. Twelve abalone were randomly selected from cultured red abalone broodstock cohort (<italic>n</italic> = 200) produced at The Cultured Abalone Farm (Goleta, CA) to initially test these methods. Following initial gonad ultrasound determination, a subset of another cohort of 5 year-old (<italic>n</italic> = 62) red abalone produced at the UC Davis Bodega Marine Laboratory were monitored using ultrasonography to detect seasonal changes in gonad size for 7 weeks. Following the 7-week assessment period, the full cohort of 5-year old abalone (<italic>n</italic> = 122) were then spawned and gonads were also evaluated using ultrasound technology before and immediately after spawning. Ultrasound imaging technology accurately differentiated between digestive and reproductive tissues, allowing for the characterization of an ultrasound gonad index score for rapid assessment. Mean gonad thickness determined via ultrasound imaging was categorized on a scale of 1–5, with an index of 1 being the lowest (gonad tissue thin or absent) and an index of 5 being the highest (gonad margin has increased and is compressing the digestive gland) for red abalone. Ultrasound imaging technology was successful in rendering a gonad index score that can be used to track gonad maturation over time in cultured or captive species. Tracking ultrasound gonad scores over time is a useful tool for improving abalone culture production, and increasing animal welfare by reducing handling stress associated with gonad assessments. Individual abalone that are empirically determined ready to be spawned can then be selected for broodstock. Non-invasive ultrasound technology has the potential for broad applications within abalone aquaculture to enhance both food and conservation aquaculture breeding programs.</p>