AUTHOR=Walde Marie , Camplong Cyprien , de Vargas Colomban , Baudoux Anne-Claire , Simon Nathalie TITLE=Viral infection impacts the 3D subcellular structure of the abundant marine diatom Guinardia delicatula JOURNAL=Frontiers in Marine Science VOLUME=9 YEAR=2023 URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2022.1034235 DOI=10.3389/fmars.2022.1034235 ISSN=2296-7745 ABSTRACT=
Viruses are key players in marine ecosystems where they infect abundant marine microbes. RNA viruses are emerging as key members of the marine virosphere. They have recently been identified as a potential source of mortality in diatoms, a group of microalgae that accounts for roughly 40% of the primary production in the ocean. Despite their likely importance, their impacts on host populations and ecosystems remain difficult to assess. In this study, we introduce an innovative approach that combines automated 3D confocal microscopy with quantitative image analysis and physiological measurements to expand our understanding of viral infection. We followed different stages of infection of the bloom-forming diatom