AUTHOR=Guo Zhijie , Shen Chenghao , Liang Haiying , Zhang Meizhen , Liang Bidan , Zhang Bin 

TITLE=Immune characterization and expression analysis of a goose-type lysozyme gene from Pinctada fucata martensii

JOURNAL=Frontiers in Marine Science

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2022.1012323

DOI=10.3389/fmars.2022.1012323

ISSN=2296-7745

ABSTRACT=<p>In the present study, a g-type lysozyme was successfully screened and cloned from <italic>Pinctada fucata martensii</italic> (designated as <italic>PmlysG</italic>). The cDNA has a length of 973 bp with an open reading frame (ORF) of 769 bp, encoding a protein of 255 amino acids. The <italic>PmlysG</italic> transcript was detected in multiple tissues by quantitative real-time PCR (qRT-PCR), with the highest expression being in the hepatopancreas. Additionally, the temporal expression of <italic>PmlysG</italic> mRNA in the hepatopancreas after <italic>in vivo</italic> stimulation with pathogen-associated molecular patterns (PAMPs), such as lipopolysaccharide (LPS), peptidoglycan (PGN) and polyinosinic acid (PolyI:C) was detected by qRT-PCR. Although <italic>PmlysG</italic> responded to all three stimulation modes, it rapidly responded to PGN stimulation. Meanwhile, the recombinant protein of g-type lysozyme of <italic>P.f. martensii</italic> (rPmlysG) was used for antibacterial function analysis, and the results showed that rPmlysG has antibacterial function against <italic>Vibrio parahaemolyticus</italic>, <italic>Aeromonas hydrophila</italic>, and <italic>Pseudomonas aeruginosa</italic>. Overall, these study results suggest that the identified PmlysG participates in the innate immune responses of <italic>P.f. martensii</italic> against pathogen infection.</p>