AUTHOR=Ma He , Liu Songtao , Wei Pingping , Fei Fan , Ren Jilong , Dai Mingyun , Ma Zhen , Liu Ying 

TITLE=Effects of LED spectrum on circadian rhythmic expression of clock genes and Aanat2 in the brain of juvenile European seabass (Dicentrarchus labrax)

JOURNAL=Frontiers in Marine Science

VOLUME=9

YEAR=2023

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2022.1005352

DOI=10.3389/fmars.2022.1005352

ISSN=2296-7745

ABSTRACT=<p>The circadian rhythm is a physiological phenomenon that occurs in various organisms with a cycle of about 24 hours. Light is one of the important environmental factors affecting biological rhythm. To clarify whether a shift in light spectrum can influence the circadian expression in fish brain, a total of 175 European seabasses [body weight: 32.5 ± 0.71) g; body length: (13.78 ± 0.35) cm] were exposed to white light (WL), red light (RL), yellow light (YL), green light (GL) or blue light (BL). After 50 days of exposure, circadian expressions of four core clock genes (<italic>Clock, Bmal1, Per2, Cry1</italic>) and <italic>Aanat2</italic> gene in brain were examined. The results showed that the temporal expression patterns of positive clock gens (<italic>Clock</italic> and <italic>Bmal1</italic>) showed increases during the scotophase and decreases during the photophase, with peaks near the middle of the darkness. <italic>Clock</italic> gene expression showed a stable circadian rhythm (R<sup>2</sup> = 0.578-0.824, <italic>P</italic>=0.000- 0.027) in all light groups while <italic>Bmal1</italic> showed circadian rhythm in WL, GL and RL, not in BL and YL. Daily expression patterns of the negative clock genes oscillated in the opposite phase from the positive clock genes, showing increasing mRNA levels during the light, decreases during the dark, and peaks near the shift from night to day, except <italic>Per2</italic> in RL and <italic>Cry1</italic> in BL. Compared with WL, the acrophases of <italic>Clock</italic> and <italic>Bmal1</italic> were delayed under all light treatments (BL: + 3.7h, +6.73h; RL: +2.4h,+1.35h; YL: + 4.94h, 2.00h; GL: +0.05, +0.16h). <italic>Cry1</italic> showed advanced acrophase under all light treatments (BL: -10.74 h, GL: -3.81 h, RL: -3.93 h, YL: -7.56 h) but <italic>Per2</italic> showed delayed acrophase in all light treatments (GL: +0.86 h, RL: +10.35 h, YL: +9.62 h), except in BL (-0.43 h). The acrophase of <italic>Aanat2</italic> was advanced by all monochromatic light, the <italic>Aanat2</italic> level was significantly increased in RL compared with other light treatment. Therefore, the results indicate that RL may regulate the expression of <italic>Aanat2</italic> gene by affecting the expression of clock gene in fish brain. Spectrum can affect the biological clock system of fish, and unreasonable spectrum may disturb the rhythm of gene expression of biological clock of fish. Under the irradiation of light spectrum, some clock genes still maintain obvious circadian oscillation, while the rhythm of some clock genes is not obvious and may be destroyed. Our findings suggest a primary role of light spectrum information to the fish brain circadian system.</p>