AUTHOR=Yin Hao-Chan , Sui Ji-Kang , Han Tian-Li , Liu Tian-Zhong , Wang Hui 

TITLE=Integration Bioprocess of B-Phycoerythrin and Exopolysaccharides Production From Photosynthetic Microalga Porphyridium cruentum

JOURNAL=Frontiers in Marine Science

VOLUME=8

YEAR=2022

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2021.836370

DOI=10.3389/fmars.2021.836370

ISSN=2296-7745

ABSTRACT=<p>Red microalga <italic>Porphyridium cruentum</italic> has great potential for converting CO<sub>2</sub> into high-value bioactive compounds, such as B-phycoerythrin (B-PE) and extracellular polysaccharides or exopolysaccharides (EPS). This study aimed to establish the integration bioprocess of B-PE and EPS production from <italic>P. cruentum</italic>. First, different kinds of growth medium and CO<sub>2</sub> concentration were assessed indoor in terms of high biomass and B-PE and EPS contents. As follows, <italic>P. cruentum</italic> cells were outdoor scale-up cultured in 700 L pressurized tubular reactors for 9 days till the biomass reached 0.85 g/L and then separated from supernatants <italic>via</italic> centrifugation. Three different methods were adopted to extract phycobiliproteins, and the highest PE contents were extracted from cells by repeated freeze-thawing treatment along with the optimization of significant variables, and finally, 7.99 mg/L B-PE (16,500 Da) with a purity index of 0.82 was obtained. Moreover, analysis of physicochemical properties of EPS extracted from <italic>P. cruentum</italic> showed that the sulfate content was 14.85% and the uronic acid content was 9.36%.</p>