AUTHOR=Zhang Tian-Shu , Zhang Ya-Ning , Lu De-Chen , Gong Ya , Du Zong-Jun 

TITLE=Carboxylicivirga marinus sp. nov., Isolated From Marine Sediment, and Genome Insight of the Genus Carboxylicivirga

JOURNAL=Frontiers in Marine Science

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2021.731212

DOI=10.3389/fmars.2021.731212

ISSN=2296-7745

ABSTRACT=<p>A Gram-stain-negative, facultatively aerobic, beige and slender bacterium, designed N1Y132<sup>T</sup>, was isolated from a sediment sample taken from coastal zone of Weihai, China (37°34′31.44″ N, 122°9′15″ E). Growth occurred at 15-33°C (optimal 25–28°C), at pH 6.5–8.5 (optimal pH 7.5), in 0–9% (w/v) NaCl (optimal 2.0–2.5%). According to the phylogenetic analysis based on the 16S rRNA gene sequences, strain N1Y132<sup>T</sup> showed the highest sequence similarity (97.9%) with <italic>Carboxylicivirga taeanensis</italic> JCM 19490<sup>T</sup>, followed by <italic>C. sediminis</italic> JR1<sup>T</sup> (97.4%), <italic>C. mesophila</italic> JCM 18290<sup>T</sup> (96.4%) and <italic>C. linearis</italic> FB218<sup>T</sup> (95.1%). Thus, strain N1Y132<sup>T</sup> was a member of the genus <italic>Carboxylicivirga.</italic> The average nucleotide identity (ANI) values between strain N1Y132<sup>T</sup> and <italic>C. sediminis</italic> JR1<sup>T</sup>, <italic>C. linearis</italic> FB218<sup>T</sup> and <italic>C. mesophila</italic> JCM 18290<sup>T</sup> were 74.8%, 71.1%, and 74.9%, respectively. Chemotaxonomic analysis showed that the sole respiratory quinone was MK-7 and the major fatty acids (&gt; 5.0%) included iso-C<sub>15</sub><sub>:</sub><sub>0</sub>, anteiso-C<sub>15</sub><sub>:</sub><sub>0</sub>, iso-C<sub>13</sub><sub>:</sub><sub>0</sub>, iso-C<sub>14</sub><sub>:</sub><sub>0</sub>, iso-C<sub>15</sub><sub>:</sub><sub>0</sub> 3-OH, and C<sub>15</sub><sub>:</sub><sub>1</sub>ω6<italic>c</italic>. The polar lipids were consisted of a phosphatidylethanolamine, two phosphoaminolipids and six unidentified lipids. The DNA G + C content was 38.1 mol%. Based on the evidence presented in this study, strain N1Y132<sup>T</sup> represents a novel species of the genus <italic>Carboxylicivirga</italic>, for which the name <italic>Carboxylicivirga marinus</italic> sp. nov. is proposed. The type strain is N1Y132<sup>T</sup> (= KCTC 72934<sup>T</sup> = MCCC 1H00431<sup>T</sup>).</p>