AUTHOR=Ouyang Liao , Liu Yan , Chen Huirong , Zaynab Madiha , Yang Xuewei , Wang Shilin , Li Shuangfei 

TITLE=Encapsulation and Algicidal Properties of Fermentation Products From Vibrio brasiliensis H115

JOURNAL=Frontiers in Marine Science

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2021.676913

DOI=10.3389/fmars.2021.676913

ISSN=2296-7745

ABSTRACT=<p>Algicidal bacteria offer an eco-friendly and promising approach for controlling harmful algae blooms (HABs). In this study, repeated batch fermentation of immobilized algicidal bacterium <italic>Vibrio brasiliensis</italic> H115 was preformed to enhance the productivity of the algicidal compounds. The highest algicidal efficiency of the fermentation products against <italic>Akashiwo sanguinea</italic> (100%) was achieved when the fermentation time was decreased from 24 to 14 h. The cell-free fermentation broth was then spray-dried and floating microcapsules were prepared from the dried powder. The optimum preparation conditions for floating microcapsules were: sodium alginate (SA), 3%; CaCO<sub>3</sub>: SA (mass ratio), 3:4; CaCl<sub>2</sub>, 3%; citric acid, 4%; ethylcellulose, 2%; crosslinking time, 30 min. Under the optimal conditions, the floating microcapsules displayed efficient <italic>A. sanguinea</italic> cell lysis ability and the algicidal efficiency increased from 10.62% (4 h) to 100% (24 h). These results suggest that the floating microcapsules could potentially be practically used for controlling the outbreaks of <italic>A. sanguinea</italic>.</p>